Regulatory

Part:BBa_K5211019:Design

Designed by: Niexin Zhou   Group: iGEM24_CHINA-HUBU-WUHAN   (2024-09-30)


The three genes, tesB, phaA and phaB, were concatenated to obtain the T1AB operon using the RBS-10 K


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The three genes, tesB, phaA and phaB, were concatenated to obtain the T1AB operon using the RBS-10 Ksequence, and the expression of the genes was regulated by the Ptet inducible promoter. （tesB derived from the E. coli K12 and phaA, phaB derived from C. necator H16)



Source

The three genes, tesB, phaA and phaB, were concatenated to obtain the T1AB operon using the RBS-10 Ksequence, and the expression of the genes was regulated by the Ptet inducible promoter. （tesB derived from the E. coli K12 and phaA, phaB derived from C. necator H16)


References